Traditional methods of extracting DNA from tissues, such as skin, mucous membranes, internal organs of animals, as well as vegetal or fungal tissues, use proteinase K as a digestive enzyme. This enzyme has a very high cost and needs specific storage care. These factors limit the number of replications of the extraction process, due to the associated high cost.
The proposed technology offers a process for extracting DNA from organic tissues using pancreatin, which is abundant and low cost, thus allowing reproduction of assays countless times. In addition to these advantages, pancreatin can be stored for a longer period of time in a simplified way, contributing to cost minimization. The developed methodology can easily compose DNA extraction kits, making it a commercially viable product.
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